Mathematical model to reduce loop mediated isothermal amplification (LAMP) false‐positive diagnosis
نویسندگان
چکیده
منابع مشابه
[Diagnosis of protozoa by loop-mediated isothermal amplification: (LAMP)].
Plasmodium, Isospora, Toxoplasma, Babesia and Cryptosporidium are parasites which have a significant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan. Classical methods are not adequate for...
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The loop-mediated isothermal amplification method (LAMP) is a recently developed molecular technique that amplifies nucleic acid under isothermal conditions. For malaria diagnosis, 150 blood samples from consecutive febrile malaria patients, and healthy subjects were screened in Thailand. Each sample was diagnosed by LAMP, microscopy and nested polymerase chain reaction (nPCR), using nPCR as th...
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As a result of the importance of quick diagnosis of Neurocryptococcosis in patients, generally, molecular techniques are preferred rather than traditional methods for their rapidity. In this research, we made an effort to design and optimize Loop mediated isothermal AMPlification (LAMP) technique using specific primers for ura5 as the target gene for detection of Cryptococcus neoformans and Cry...
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We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole ...
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Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2019
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.201900167